PY17X_1356700 calcium-dependent protein kinase 5 (CDPK5)
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. berghei ANKA |
Refractory |
RMgm-536 | Imported from RMgmDB | |
| P. berghei ANKA |
Refractory |
PlasmoGEM (Barseq) | PlasmoGEM | |
| P. falciparum 3D7 |
Refractory |
20466936 (Conditional) | Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Refractory |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. falciparum 3D7 | Asexual |
Egress defect |
29487234 (Knock down)
Micronemes fail to discharge |
Theo Sanderson, Wellcome Trust Sanger Institute |
| P. falciparum 3D7 | Asexual |
Cell cycle arrest |
28288121 (Conditional)
Knock sideways to the nucleus caused accumulation of segmented schizonts |
Theo Sanderson, Wellcome Trust Sanger Institute |
| P. falciparum 3D7 | Asexual |
Egress defect |
20466936 (Knock down) | Theo Sanderson, Francis Crick Institute |
| P. falciparum 3D7 | Asexual |
Difference from wild-type |
31915223 (Knock down)
'58 phosphorylation sites on 50 proteins with significant reduction in levels of PfCDPK5-deficient parasites. Furthermore, gene ontology analysis of the identified proteins reveals enrichment in transmembrane- and membrane-associated proteins and in proteins associated with transport activity. Among the identified proteins is PfNPT1, a member of the apicomplexan-specific novel putative transporter (NPT) family of proteins.' |
Theo Sanderson, Francis Crick Institute |
Imaging data (from Malaria Metabolic Pathways)
Localization and KS with protein targets. (a,b) Knockin cell lines of CDPK5-2×FKBP-GFP with an mCherry-tagged nuclear mislocalizer (MislocalizerN) (a) and HP1-2×FKBP-GFP with a plasma-membrane mislocalizer (MislocalizerP). In synchronized parasites, KS of CDPK5 caused an accumulation of segmented schizonts. (b). Representative fluorescence microscopy images of rapalog-treated and control cells (after 16 h). Mislocalization of HP1 caused an accumulation of gametocytes.Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T. A genetic system to study Plasmodium falciparum protein function. Nat Methods. 2017 Mar 13.
See original on MMPMore information
| PlasmoDB | PY17X_1356700 |
| GeneDB | PY17X_1356700 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | null |
| Orthologs | PBANKA_1351500 , PCHAS_1356100 , PF3D7_1337800 , PKNH_1263500 , PVP01_1216900 , PVX_082820 |
| Google Scholar | Search for all mentions of this gene |