Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
RMgm-536 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
20466936 (Conditional) Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Egress defect
29487234 (Knock down)
Micronemes fail to discharge
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Asexual
Cell cycle arrest
28288121 (Conditional)
Knock sideways to the nucleus caused accumulation of segmented schizonts
Theo Sanderson, Wellcome Trust Sanger Institute

Imaging data (from Malaria Metabolic Pathways)

Localization and KS with protein targets. (a,b) Knockin cell lines of CDPK5-2×FKBP-GFP with an mCherry-tagged nuclear mislocalizer (MislocalizerN) (a) and HP1-2×FKBP-GFP with a plasma-membrane mislocalizer (MislocalizerP). In synchronized parasites, KS of CDPK5 caused an accumulation of segmented schizonts. (b). Representative fluorescence microscopy images of rapalog-treated and control cells (after 16 h). Mislocalization of HP1 caused an accumulation of gametocytes.Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T. A genetic system to study Plasmodium falciparum protein function. Nat Methods. 2017 Mar 13.

See original on MMP

More information

PlasmoDB PKNH_1263500
GeneDB PKNH_1263500
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK13_3750c, PKH_121600
Orthologs PBANKA_1351500 , PCHAS_1356100 , PF3D7_1337800 , PVP01_1216900 , PVX_082820 , PY17X_1356700
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