Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
RMgm-536 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
20466936 (Conditional) Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Egress defect
29487234 (Knock down)
Micronemes fail to discharge
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Asexual
Cell cycle arrest
28288121 (Conditional)
Knock sideways to the nucleus caused accumulation of segmented schizonts
Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7 Asexual
Egress defect
20466936 (Knock down) Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Asexual
Difference from wild-type
31915223 (Knock down)
'58 phosphorylation sites on 50 proteins with significant reduction in levels of PfCDPK5-deficient parasites. Furthermore, gene ontology analysis of the identified proteins reveals enrichment in transmembrane- and membrane-associated proteins and in proteins associated with transport activity. Among the identified proteins is PfNPT1, a member of the apicomplexan-specific novel putative transporter (NPT) family of proteins.'
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Localization and KS with protein targets. (a,b) Knockin cell lines of CDPK5-2×FKBP-GFP with an mCherry-tagged nuclear mislocalizer (MislocalizerN) (a) and HP1-2×FKBP-GFP with a plasma-membrane mislocalizer (MislocalizerP). In synchronized parasites, KS of CDPK5 caused an accumulation of segmented schizonts. (b). Representative fluorescence microscopy images of rapalog-treated and control cells (after 16 h). Mislocalization of HP1 caused an accumulation of gametocytes.Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T. A genetic system to study Plasmodium falciparum protein function. Nat Methods. 2017 Mar 13.

See original on MMP

More information

PlasmoDB PBANKA_1351500
GeneDB PBANKA_1351500
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PB000275.01.0, PB000341.03.0, PBANKA_135150
Orthologs PCHAS_1356100 , PF3D7_1337800 , PKNH_1263500 , PVP01_1216900 , PVX_082820 , PY17X_1356700
Google Scholar Search for all mentions of this gene