Last updated 3 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-4777 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1169 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1168 Imported from RMgmDB
P. berghei ANKA
Refractory
RMgm-193 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
26991313 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-4777 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
Difference from wild-type
RMgm-4777
reduced production of male gametocytes; (somewhat) reduced exflagellation
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1169
A reduced growth rate of asexual blood stages (mean multiplication rate of 6.8x per 24 hour in the cloning assay; wild type parasites show a multiplication rate of 10x per 24 hour in this assay)
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1168
A reduced growth rate of asexual blood stages (mean multiplication rate of 6.8x per 24 hour in the cloning assay; wild type parasites show a multiplication rate of 10x per 24 hour in this assay)
Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-1169 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-1168 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1169 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1168 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1169
Reduced numbers of (mature) oocysts
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1168
Reduced numbers of (mature) oocysts
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1169
Reduced numbers of (mature) oocysts.Stronly reduced numbers (>10 times) of salivary gland sporozoites
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1168
Reduced numbers of (mature) oocysts.Stronly reduced numbers (>10 times) of salivary gland sporozoites
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1169
Strongly reduced numbers (>10 times) of salivary gland sporozoites. However, sporozoites show normal infectivity to mice. Intravenous injection of 10.000 mutant sporozoites resulted in blood infections in mice (n=3) with a prepatent period (5-6 days) that is comparable to the prepatent period of wild type parasites.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1168
Strongly reduced numbers (>10 times) of salivary gland sporozoites. However, sporozoites show normal infectivity to mice. Intravenous injection of 10.000 mutant sporozoites resulted in blood infections in mice (n=3) with a prepatent period (5-6 days) that is comparable to the prepatent period of wild type parasites.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Localization of PfMRP2 and PfMDR5 within the asexual stages of P. falciparum. In column 1 blue (DAPI) shows the nuclear staining. In column 2 red shows PfMRP2 staining. In column 3 green shows glycophorin-A (A and B) or PfERC (C and D) staining. Column 4 shows the merge of column 1–3. Finally, column 5 shows the differential interference contrast image. Represented in rows: A, multiple infected erythrocyte: rings; B, double infected erythrocyte: trophozoites; C, three trophs (T) and two rings (R); D, early schizont. Arrow indicates food vacuole. Both transporters are localized to the plasma membrane in all asexual stages of the parasite.Kavishe RA, van den Heuvel JJ, van de Vegte-Bolmer M, Luty AJ, Russel FG, Koenderink JB. Localization of the ATP-binding cassette (ABC) transport proteins PfMRP1, PfMRP2, and PfMDR5 at the Plasmodium falciparum plasma membrane. Malar J. 2009 8:205.

See original on MMP

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