PfAKAL localization in P. falciparum merozoites by indirect immunofluorescence assays. Images with PfAKAL localization (green), PfAMA1 localization (red), Hoechst DNA staining (blue) and differential interference contrast (DIC) and were taken. a P. falciparum 3D7 egressing merozoites, b P. falciparum 3D7 invading merozoite. The bars represent 2 μm. PfAKAL is expressed in egressing (a) and invading (b) merozoites.Bandje K, Naissant B, Bigey P, Lohezic M, Vayssières M, Blaud M, Kermasson L, Lopez-Rubio JJ, Langsley G, Lavazec C, Deloron P, Merckx A. Characterization of an A-kinase anchoring protein-like suggests an alternative way of PKA anchoring in Plasmodium falciparum. Malar J. 2016 15(1):248.

PfAKAL subcellular localizations in asexual and sexual P. falciparum blood stages. Images with PfAKAL localization (green), Hoechst DNA staining (blue), differential interference contrast (DIC) were taken on rings a trophozoites, b schizonts, c as well as on stage II, d stage III, e stage IV, f and stage V, g gametocytes, infected erythrocytes. “Bright orange” on DIC images indicates haemozoin crystals. The bars represent 2 μm. In asexual parasites PfAKAL was undetectable in ring stages (a), with expression predominantly in late trophozoites and schizonts. Interestingly, a diffuse subcellular localization of PfAKAL was observed in the parasite cytoplasm and in the nucleus in trophozoite stages, whereas it was mostly nuclear in schizonts (b, c). In sexual stages, PfAKAL was also detected in the cytoplasm of the parasite, although it mainly accumulates around the nucleus.Bandje K, Naissant B, Bigey P, Lohezic M, Vayssières M, Blaud M, Kermasson L, Lopez-Rubio JJ, Langsley G, Lavazec C, Deloron P, Merckx A. Characterization of an A-kinase anchoring protein-like suggests an alternative way of PKA anchoring in Plasmodium falciparum. Malar J. 2016 15(1):248.