Mutants developed gametocytes and formed ookinetes in vitro in similar numbers to, and morphologically indistinguishable from, wild type parasites in Giemsa-stained preparations. Reduced numbers of oocysts (reduction of 60-85%) are produced in A. stephensi mosquitoes.

Normal numbers of ookinetes are produced. Reduced numbers of oocysts (reduction of 60-85%) are produced in A. stephensi mosquitoes. Although oocyst numbers were markedly reduced in mutant-infected mosquitoes, those oocysts that developed were morphologically indistinguishable from wild type oocysts of comparable age, and formed large numbers of sporozoites similar to wild type oocysts.

Mutant sporozoites appeared morphologically normal and successfully invaded the salivary glands. Mosquitoes that were infected with mutant sporozoites successfully transmitted the parasite to mice.

Mutant sporozoites appeared morphologically normal and successfully invaded the salivary glands. Mosquitoes that were infected with mutant sporozoites successfully transmitted the parasite to mice.