Normal production of ookinetes. Reduced ookinete invasion and traversal of the midgut wall (see also 'Additional remarks phenotype').

Strong reduction of oocyst formation (0% of wild type). Only two oocysts were observed in a total of 130 mosquitoes.

No sporozoites are produced. No tramission to C57BL/6 mice by mosquito bite.When ookinetes were injected directly into the mosquito hemocoel to bypass the midgut barrier mosquito infectivity was rescued as assayed by the quantification of salivary gland sporozoites. The development of oocysts in the hemocoel is thus not affected. The formed sporozoites were also fully infectious to mice by mosquito-bite.

When ookinetes were injected directly into the mosquito hemocoel to bypass the midgut barrier mosquito infectivity was rescued as assayed by the quantification of salivary gland sporozoites. The development of oocysts in the hemocoel is thus not affected. The formed sporozoites were also fully infectious to mice by mosquito-bite.