Localization of PfCPO by immunofluorescence microscopy. Localization of PfCPO with Mitotracker green (mitochondrial marker), PfALAS (mitochondrial marker) and PfUROD (apicoplast marker) was studied. IE, infected erythrocyte; P, parasite; H, hemozoin. Scale bar=5 μm. results presented in Fig. 4 indicate that the PfCPO signal was distributed throughout the parasite, but did not colocalize with that of Mitotracker dye (mitochondrial marker), PfALAS (mitochondrial marker) or PfUROD (apicoplast marker).Nagaraj VA, Prasad D, Arumugam R, Rangarajan PN, Padmanaban G. Characterization of coproporphyrinogen III oxidase in Plasmodium falciparum cytosol. Parasitol Int. 2010 59:121-127. Copyright Elsevier 2011

Localization of PfCPO by immunofluorescence microscopy. Localization of PfCPO with Mitotracker green (mitochondrial marker), PfALAS (mitochondrial marker) and PfUROD (apicoplast marker) was studied. IE, infected erythrocyte; P, parasite; H, hemozoin. Scale bar=5 μm. results presented in Fig. 4 indicate that the PfCPO signal was distributed throughout the parasite, but did not colocalize with that of Mitotracker dye (mitochondrial marker), PfALAS (mitochondrial marker) or PfUROD (apicoplast marker).Nagaraj VA, Prasad D, Arumugam R, Rangarajan PN, Padmanaban G. Characterization of coproporphyrinogen III oxidase in Plasmodium falciparum cytosol. Parasitol Int. 2010 59:121-127. Copyright Elsevier 2011

Left panel: Images of fixed 3D7 parasites expressing full-length PBGD tagged at its endogenous locus with C-terminal GFP confirm targeting of the native protein to the parasite apicoplast. Parasites were stained with αGFP and αACP (ACP, apicoplast marker).Sigala PA, Crowley JR, Henderson JP, Goldberg DE. Deconvoluting heme biosynthesis to target blood-stage malaria parasites. Elife. 2015 Jul 14;4. Right panel: Fluorescence microscopy images of live 3D7 parasites episomally expressing full-length CPO with a C-terminal GFP tag confirm protein localization to the parasite cytoplasm