No difference in gam production, exflagellation, but males are infertile due to fertilisation defect

No difference in gam production, exflagellation, but males are infertile due to fertilisation defect

Normal numbers of male and female gametocytes are produced. Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.

Normal numbers of male and female gametocytes are produced and the sex ratio was comparable to that of wild type gametocytes. Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.

Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.Mutant males gametes showed flagella motility and adhesion to surrounding erythrocytes, forming exfagellating centers comparable to wild type. However, no successful entering of flagella into female gametes was observed.

Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.Mutant males attach to females but failed to progress from adhesion to membrane fusion and fertilization.No zygotes and ookinetes are formed.

No ookinete and oocyst formation (see phenotype 'Fertilization and ookinete').

No ookinete and oocyst formation (see phenotype 'Fertilization and ookinete').

No sporozoite formation (see phenotype 'Fertilization and ookinete' and 'Oocyst').