Ookinete numbers (in vitro) reduced to 52% of wild type

Oocyst numbers (day 12-14) reduced to 20% of wild type

Normal numbers of salivary gland sporozoites are produced. Sporozoites showed reduced gliding motility and cell traversal capacity in vitro. Invasion of HepG2 cells in vitro was comparable to that of wild type parasites. Infectivity of sporozoites to mice was strongly reduced (as tested by intravenous injection of purified sporozoites or after infection of mice by mosquito bite). Salivary gland sporozoites show premature development into early liver stage forms (see further 'Additional remarks phenotype')

transmitted by mosquito bite

Sporozoites showed reduced gliding motility and cell traversal capacity in vitro. Invasion of HepG2 cells in vitro was comparable to that of wild type parasites. Infectivity of sporozoites to mice was strongly reduced (as tested by intravenous injection of purified sporozoites or after infection of mice by mosquito bite). Salivary gland sporozoites show premature development into early liver stage forms (see further 'Additional remarks phenotype')