CDPK7 may be present in the ER and vesicles. A IFA was performed to localize CDPK7 in various asexual stages of the parasite using antisera against CDPK7. R, rings; T, trophozoites; S, schizonts. B. Co-staining was done using antisera against CDPK7 and BiP on trophozoites (a) or with anti-RAP1 mAb on free merozoites (b). C. Immuno-EM performed on a trophozoite stage parasite indicated the presence of CDPK7 in ER and vesicular compartments (arrows). Various parasite organelles are indicated: FV, food vacuole; PM/PVM, parasite membrane/parasite vacuolar membrane; ER, Endoplasmic Reticulum; N, Nucleus.Kumar P, Tripathi A, Ranjan R, Halbert J, Gilberger T, Doerig C, Sharma P. Regulation of Plasmodium falciparum development by Calcium-Dependent Protein Kinase 7 (PfCDPK7). J Biol Chem. 2014 Jun 3. [Epub ahead of print]