Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
RMgm-992 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. yoelii yoelii 17X
Possible
RMgm-4781 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Refractory
24086138 (Insert. mut.)
\"We disrupted the Suf pathway using a dominant negative mutant of SufC and showed that these parasites only survive when cultured in the presence of IPP. Furthermore, these parasites lack the apicoplast organelle and its organellar genome – a phenotype not observed when isoprenoid biosynthesis was specifically inhibited with fosmidomycin. Taken together, these results demonstrate that the Suf pathway has a fundamental role in maintaining the apicoplast organelle in addition to any role in isoprenoid biosynthesis.\"
Theo Sanderson, Francis Crick Institute
P. yoelii yoelii 17X Asexual
No difference
RMgm-4781 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
Difference from wild-type
RMgm-4781
normal production of gametocytes; normal exflagellation
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Plasmodium falciparum PfSufC is targeted to the apicoplast and may be peripherally membrane-associated. (A) Immunofluorescence localisation of SufC in the Plasmodium falciparum D10 ACPleader-GFP line. SufC signal (red, a), GFP (green, b) and their overlap (c) in two trophozoite examples. Parasite nuclei stained with DAPI (d), and the overall merged figure with the phase contrast scan (e) are shown. The signals obtained for a mitochondrial marker (MitoTracker Deep Red, a), SufC (green, b) and nucleus (blue, d). SufC was not localised to the mitochondrion.Kumar B, Chaubey S, Shah P, Tanveer A, Charan M, Siddiqi MI, Habib S. Interaction between sulphur mobilisation proteins SufB and SufC: Evidence for an iron-sulphur cluster biogenesis pathway in the apicoplast of Plasmodium falciparum. Int J Parasitol. 2011 41:991-9.

See original on MMP

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