Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
25843709 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
25843709 Theo Sanderson, Wellcome Trust Sanger Institute

Imaging data (from Malaria Metabolic Pathways)

Localization of PfPDH and PfOxoDH within erythrocytes infected with 3D7 parasites. Localization of PfPDH (top panel) and PfOxoDH (bottom panel) in transgenic parasites. The left column shows phase contrast images, followed by fluorescence images of a nuclear dye (Hoechst; blue), GFP-tagged leader sequences of the target enzymes (green), a mitochondrial dye MitoTracker; red) and all four images merged together (right column). Scale bars, 2 mm. The E1-a subunit of PfPDH localizes to a discrete organelle adjacent to the mitochondrion and distinct from the nucleus, consistent with an apicoplast localization (top panel). The truncated E1 subunit of PfOxoDH, which has been predicted to have a mitochondrial localization,colocalizes with MitoTracker when fused to GFP (bottom panel), consistent with a mitochondrial localization.Chan XW, Wrenger C, Stahl K, Bergmann B, Winterberg M, Müller IB, Saliba KJ. Chemical and genetic validation of thiamine utilization as an antimalarial drug target. Nat Commun. 2013 Jun 27;4:2060.

See original on MMP

Localization of PfPDH and PfOxoDH within erythrocytes infected with 3D7 parasites. Localization of PfPDH (top panel) and PfOxoDH (bottom panel) in transgenic parasites. The left column shows phase contrast images, followed by fluorescence images of a nuclear dye (Hoechst; blue), GFP-tagged leader sequences of the target enzymes (green), a mitochondrial dye MitoTracker; red) and all four images merged together (right column). Scale bars, 2 mm. The E1-a subunit of PfPDH localizes to a discrete organelle adjacent to the mitochondrion and distinct from the nucleus, consistent with an apicoplast localization (top panel). The truncated E1 subunit of PfOxoDH, which has been predicted to have a mitochondrial localization,colocalizes with MitoTracker when fused to GFP (bottom panel), consistent with a mitochondrial localization.Chan XW, Wrenger C, Stahl K, Bergmann B, Winterberg M, Müller IB, Saliba KJ. Chemical and genetic validation of thiamine utilization as an antimalarial drug target. Nat Commun. 2013 Jun 27;4:2060.

See original on MMP

More information

PlasmoDB PKNH_1314700
GeneDB PKNH_1314700
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK8_2230w, PKH_051030
Orthologs PBANKA_0710100 , PCHAS_0719200 , PF3D7_0820700 , PVP01_0513600 , PVX_089325 , PY17X_0710300
Google Scholar Search for all mentions of this gene