Last updated 2 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Refractory
32552779
Initial attempt to generate frameshift with CRISPR failed
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
32552779 (Knock down)
"The growth of more than 50% of the PfArp4-Ty1-Ribo parasites was arrested when the PfArp4 protein was downregulated". "Consistent with the results of the growth curve analysis, western blot (WB) analysis showed that the expression level of the PfArp4 protein was significantly reduced in the trophozoite stage parasites when GlcN was present in the culture (Fig. 1e). An abnormal morphology was also observed in the trophozoite-stage parasites of the PfArp4-Ty1-Ribo line treated with GlcN after one cell cycle".
Theo Sanderson, Francis Crick Institute

More information