Localization of PfSmD1, SmD2 and SmD3 at the asexual blood stages of Plasmodium falciparum. Stage-specific expression of Plasmodium Sm proteins at asexual blood stages; ring (8 hr), trophozoite (36 hr) and schizont (42 hr) stages by immuno-localization. Immuno-fluorescence assay were performed using (A) anti mouse-PfSmD1, (B) anti- PfSmD2 and (C) anti-PfSmD3 antisera (1:50 dilution) on thin blood smears of P. falciparum. PfSm proteins showed nucleo-cytoplasmic localization. Hossain M, Sharma S, Korde R, Kanodia S, Chugh M, Rawat K, Malhotra P. Organization of Plasmodium falciparum spliceosomal core complex and role of arginine methylation in its assembly. Malar J. 2013 Sep 18;12(1):333.

Upper panel: Co-localization of PfTudor-SN and Histone h2A. Immunofluorescence assays were performed at P. falciparum schizont stage using peptide antibodies generated against Histone h2A. The interacting h2A in the parasite were co-localized with anti-PfTudor-SN antibody in the nucleus.Lower panel: .Co-localization of PfTudor-SN and SmD1 protein of the parasite. PfTudor-SN was also co-localized with the PfSmD1 suggesting a close association between themHossain MJ, Korde R, Singh PK, Kanodia S, Ranjan R, Ram G, Kalsey GS, Singh R, Malhotra P. Plasmodium falciparum Tudor Staphylococcal Nuclease interacting proteins suggest its role in nuclear as well as splicing processes. Gene. 2010 468:48-57. Copyright Elsevier 2011