Last updated 6 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Egress defect
31075098 (Conditional)
Delayed egress
Theo Sanderson, Google AI
P. falciparum 3D7 Asexual
Invasion defect
31075098 (Conditional) Theo Sanderson, Google AI

Imaging data (from Malaria Metabolic Pathways)

Expression of PfACb in P. falciparum blood stages. Immunofluorescence assays (IFA) were used to detect PfACb (green) in P. falciparum rings (i), trophozoites (ii), schizonts (iii) and merozoites (iv) using mouse antisera against a peptide derived from PfACb. Nuclear DNA was stained with DAPI (blue). Rabbit antiserum against P. falciparum cytoplasmic protein PfNAPL (red) was used for co-localization. Yellow indicates overlap of PfACb and PfNAPL. PfACb is thus expressed in P. falciparum merozoites and is localized in the cytoplasm.Dawn A, Singh S, More KR, Siddiqui FA, Pachikara N, Ramdani G, Langsley G, Chitnis CE. The Central Role of cAMP in Regulating Plasmodium falciparum Merozoite Invasion of Human Erythrocytes. PLoS Pathog. 2014 18;10(12):e1004520.

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Upper panel: Location of Dansyl-MAS 7 and MAS 17 peptides, within the parasite infected erythrocyte. Parasite nuclei are shown in blue, Dansyl fluorescence in green and BODIPY-TR-Ceramide in red. A merge and bright field (BF) image are shown. Scale bar = 3 μm. MAS-7 probably binds to adenylyl cyclase .Lower panel: Location of Dansyl-MAS 7 peptide, within the parasite infected erythrocyte following 1 hour (upper panel) and 2 hour (lower panel) incubation. Scale bar = 3 μm.Peatey CL, Dixon MW, Gardiner DL, Trenholme KR. Temporal evaluation ofcommitment to sexual development in Plasmodium falciparum. Malar J. 2013 12:134.

See original on MMP

Upper panel: Location of Dansyl-MAS 7 and MAS 17 peptides, within the parasite infected erythrocyte. Parasite nuclei are shown in blue, Dansyl fluorescence in green and BODIPY-TR-Ceramide in red. A merge and bright field (BF) image are shown. Scale bar = 3 μm. MAS-7 probably binds to adenylyl cyclase .Lower panel: Location of Dansyl-MAS 7 peptide, within the parasite infected erythrocyte following 1 hour (upper panel) and 2 hour (lower panel) incubation. Scale bar = 3 μm.Peatey CL, Dixon MW, Gardiner DL, Trenholme KR. Temporal evaluation ofcommitment to sexual development in Plasmodium falciparum. Malar J. 2013 12:134.

See original on MMP

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