PCHAS_1361300 kelch protein K13, putative
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. falciparum 3D7 |
Refractory |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. falciparum 3D7 | Asexual |
Cell cycle arrest |
28288121 Knock sideways as well as diCre depletion led to arrest at rings and
eventually condensed forms |
Theo Sanderson, Wellcome Trust Sanger Institute |
Imaging data (from Malaria Metabolic Pathways)
Analysis of the artemisinin-resistance proteins Kelch13. (a) Localization of native N-terminally GFP-tagged Kelch13 (GFP-2×FKBP-Kelch13). The endogenously expressed GFP-2×FKBP-Kelch13 was located in foci in the parasite cytoplasm (a). At least one of these foci was usually close to the parasite food vacuole. (c) KS with the 2×FKBP-GFP-2×FKBP-Kelch13 parasite line. Left, fluorescence microscopy images of live cells, showing the KS (rapalog) and control. KS with this cell line (Supplementary Figs. 1 and 9b) led to complete mislocalization of Kelch13 and to a rapid-growth phenotype.Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T. A genetic system to study Plasmodium falciparum protein function. Nat Methods. 2017 Mar 13.
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| PlasmoDB | PCHAS_1361300 |
| GeneDB | PCHAS_1361300 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | PC000096.03.0, PC000355.03.0, PCAS_136130, PCHAS_136130 |
| Orthologs | PBANKA_1356700 , PF3D7_1343700 , PKNH_1257700 , PVP01_1211100 , PVX_083080 , PY17X_1362400 |
| Google Scholar | Search for all mentions of this gene |