Last updated 7 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Cell cycle arrest
28288121
Knock sideways as well as diCre depletion led to arrest at rings and eventually condensed forms
Theo Sanderson, Wellcome Trust Sanger Institute

Imaging data (from Malaria Metabolic Pathways)

Analysis of the artemisinin-resistance proteins Kelch13. (a) Localization of native N-terminally GFP-tagged Kelch13 (GFP-2×FKBP-Kelch13). The endogenously expressed GFP-2×FKBP-Kelch13 was located in foci in the parasite cytoplasm (a). At least one of these foci was usually close to the parasite food vacuole. (c) KS with the 2×FKBP-GFP-2×FKBP-Kelch13 parasite line. Left, fluorescence microscopy images of live cells, showing the KS (rapalog) and control. KS with this cell line (Supplementary Figs. 1 and 9b) led to complete mislocalization of Kelch13 and to a rapid-growth phenotype.Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T. A genetic system to study Plasmodium falciparum protein function. Nat Methods. 2017 Mar 13.

See original on MMP

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