Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-1114 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-970 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1114
Asexual blood infections in mice induced infections with a delayed increase of the parasitemia
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-970 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-1114 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1114 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-1114 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-970 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1114
Normal numbers of oocyst-derived (hemocoel) sporozoites are formed. Sporozoites have greatly impaired gliding motility and capacity to invade salivary glands.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-970
Only 'hemocoel-associated' sporozoites are formed. Sporozoites do not invade salivary glands. Hemocoel sporozoites show reduced gliding. Sporozoites are not infectious to hepatocytes in culture.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1114
Normal numbers of oocyst-derived (hemocoel) sporozoites are formed. Sporozoites have greatly impaired gliding motility and capacity to invade salivary glands. Sporozoites are not able to invade hepatocytes in vitro. Sporozoites were not infectious to mice.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-970
Sporozoites are not infectious to hepatocytes in culture and not infectious to mice (after intravenous inoculation of hemocoel sporozoites)
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Immunoelectron microscopy. Late-schizont stage parasites were incubated with anti-falstatin antibodies and gold-conjugated second antibody and then evaluated by electron microscopy. Labels show individual merozoites (M) and erythrocyte cytosol (EC). Falstatin localizes to the periphery of rings and early schizonts, is diffusely expressed in late schizonts and merozoites, and is released upon the rupture of mature schizonts.Pandey KC, Singh N, Arastu-Kapur S, Bogyo M, Rosenthal PJ. Falstatin, a cysteine protease inhibitor of Plasmodium falciparum, facilitates erythrocyte invasion. PLoS Pathog. 2006 2(11):e117.

See original on MMP

Immunolocalization of Falstatin. Immunofluorescence microscopy. Erythrocytes infected with synchronized 3D7 or W2 parasites were collected every 8 h, stained with DAPI and anti-falstatin antibodies and FITC-second antibody, and then evaluated by immunofluorescence microscopy. Falstatin is present in schizonts, merozoites, and rings, but not in trophozoites, the stage at which the cysteine protease activity of P. falciparum is maximal. Pandey KC, Singh N, Arastu-Kapur S, Bogyo M, Rosenthal PJ. Falstatin, a cysteine protease inhibitor of Plasmodium falciparum, facilitates erythrocyte invasion. PLoS Pathog. 2006 2(11):e117.

See original on MMP

More information

PlasmoDB PCHAS_0813300
GeneDB PCHAS_0813300
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PC000277.02.0, PCAS_081280, PCHAS_081330
Orthologs PBANKA_0813000 , PF3D7_0911900 , PKNH_0709900 , PVP01_0710400 , PVX_099035
Google Scholar Search for all mentions of this gene