Normal gametocyte production and gamete formation. Evidence for normal fertilisation. Formation of retort shaped, immature ookinetes but no mature ookinete production.The majority of zygotes failed to show apical complex protrusions.

The mutant formed morphologically normal female and male gametocytes, and the male gametocytes showed normal exflagellation. Pbap2-fg2(-) parasites failed to produce banana-shaped ookinetes; more than half of the fertilized population stopped developing at round zygotes, and the others, at retort-form ookinetes.

The mutant formed morphologically normal female and male gametocytes, and the male gametocytes showed normal exflagellation. Pbap2-fg2(-) parasites failed to produce banana-shaped ookinetes; more than half of the fertilized population stopped developing at round zygotes, and the others, at retort-form ookinetes.

The mutant formed morphologically normal female and male gametocytes, and the male gametocytes showed normal exflagellation. Pbap2-fg2(-) parasites failed to produce banana-shaped ookinetes; more than half of the fertilized population stopped developing at round zygotes, and the others, at retort-form ookinetes.

No mature ookinete production. No oocyst production.

Formation of aberrant ookinetes. No oocyst/sporozoite formation

Formation of aberrant ookinetes. No oocyst/sporozoite formation

Formation of aberrant ookinetes. No oocyst/sporozoite formation

Formation of aberrant ookinetes. No oocyst/sporozoite formation

Formation of aberrant ookinetes. No oocyst/sporozoite formation

Formation of aberrant ookinetes. No oocyst/sporozoite formation

Normal numbers of gametocytes are produced. Only ookinetes with aberrant morphology are produced.

Normal numbers of gametocytes are produced. Normal exflagellation. Normal egress of gametes. Males are fertile (shown in crossing-experiments). Fertilization ability of females is impaired. Fertilized zygotes of the ap2-o3 mutants were developmentally arrested at the early stages (stage II-III), displaying incomplete elongation. Only ookinetes with aberrant morphology are produced.

Normal numbers of gametocytes are produced. Only ookinetes with aberrant morphology are produced. No oocysts and salivary gland sporozoites are formed.

Normal numbers of gametocytes are produced. Only ookinetes with aberrant morphology are produced. No oocysts and salivary gland sporozoites are formed.