(B) A merozoite was dual-labeled with anti-PfPK2 and anti-Clag3.1 PFC0110w antibodies. An overlaid image is shown in the right-hand panels. (C) The green fluorescence corresponding to PfPK2 was detected in merozoites that had just ruptured from or invaded erythrocytes (b, e, and h). The images from differential interference contrast (DIC) are shown. The nuclei were stained by TO-PRO-3 iodide (blue) (c, f, and i). Images of parasites that had just ruptured from (a–c) or were invading (d–f) erythrocytes. White arrows indicate ring-formed parasites that may have just invaded erythrocytes (g–i).Kato K, Sudo A, Kobayashi K, Tohya Y, Akashi H. Characterization of Plasmodium falciparum protein kinase 2. Mol Biochem Parasitol. 2008 162:87-95. Copyright Elsevier 2010.

Immunofluorescence assays, using specific antibodies, detected the proteins of interest (in green) in asexual blood stage (ABS) parasites, using MSP1 (in red). Nuclei were highlighted by Hoechst nuclear stain (in blue). Bar, 5 μm. Ngwa CJ, Scheuermayer M, Mair GR, Kern S, Brügl T, Wirth CC, Aminake MN, Wiesner J, Fischer R, Vilcinskas A, Pradel G. Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito. BMC Genomics. 2013 Apr 15;14:256