Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
21984828 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Localization of epitope-tagged ATP synthase subunits detected using immuno-fluoresence microscopy. Immunofluoresence of alpha, gamma, delta, epsilon, OSCP and c subunits expressed under control of the mRPL2 promoter. Expression of the beta subunit was under the control of its own promoter. Green fluorescence: Anti-HA antibody (AlexaFluor 488-conjugated secondary antibody). Red fluorescence: Mitotracker Red CM-H2XRos. Blue fluorescence: DAPI (4'-6-Diamidino-2-phenylindole). DIC: Differential interference contrast microscopy.Balabaskaran Nina P, Morrisey JM, Ganesan SM, Ke H, Pershing AM, Mather MW, Vaidya AB. ATP synthase complex of Plasmodium falciparum: dimeric assembly in mitochondrial membranes and resistance to genetic disruption. J Biol Chem. 2011 286(48):41312-22. PMID:

See original on MMP

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