Normal numbers of midgut sporozoites are formed. Sporozoites do not display gliding motility. Sporozoites are strongly affected in their capacity to invade salivary glands. In wild type infected mosquitoes the proportion of total salivary gland-associated sporozoites released from within the glands was ~80% and in mutant infected mosquitoes only ~4%. Sporozoites are also affected in their infectivity to BALB/c mice (mutant sporozoites were at least 100 times less infective than WT sporozoites).

Sporozoites are affected in their infectivity to BALB/c mice (mutant sporozoites were at least 100 times less infective than WT sporozoites).

Normal numbers of midgut sporozoites are formed. Sporozoites do not display gliding motility. Sporozoites are strongly affected in their capacity to invade salivary glands (the number of salivary glandassociated, sporozoites per infected mosquito was 34 times smaller than in wild type). Sporozoites are also affected in their infectivity to Sprague-Dawley rats (~10,000 times less infective than wild type sporozoites).

Not analysed in detail:- Normal oocyst production with sporozoite formation. Sporozoites are released in the hemocoel but are impaired in salivary gland invasion. No (strongly reduced) salivary gland sporozoite numbers.- The mutant lacks expression of TRAP. The trap gene has been deleted and does not contain a drug-selectable marker (that has been removed using negative selection with 5-FC). This mutant is used to introduce mutated forms of the trap gene.

Sporozoites are affected in their infectivity to Sprague-Dawley rats (~10,000 times less infective than wild type sporozoites).