Last updated 3 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-4774 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1387 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1166 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1165 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
26991313 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
25884516 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-4774 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
Difference from wild-type
RMgm-4774
normal production of gametocytes; normal exflagellation
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1387 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1166 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1165 Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-1387 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-1166 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-1165 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1166 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1165 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1166
Reduced numbers of (mature) oocysts
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1165
Reduced numbers of (mature) oocysts
Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1387 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1166
Reduced numbers of (mature) oocysts.Stronly reduced numbers (>10 times) of salivary gland sporozoites
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1165
Reduced numbers of (mature) oocysts.Stronly reduced numbers (>10 times) of salivary gland sporozoites
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1387
Delayed transmission by bite of infected mosquitoes
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1166
Stron9ly reduced numbers (>10 times) of salivary gland sporozoites. However, sporozoites show normal infectivity to mice. Intravenous injection of 10.000 mutant sporozoites resulted in blood infections in mice (n=3) with a prepatent period (5-6 days) that is comparable to the prepatent period of wild type parasites.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1165
Strongly reduced numbers (>10 times) of salivary gland sporozoites. However, sporozoites show normal infectivity to mice. Intravenous injection of 10.000 mutant sporozoites resulted in blood infections in mice (n=3) with a prepatent period (5-6 days) that is comparable to the prepatent period of wild type parasites.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Indirect immunofluorescence of W2 infected erythrocytes with anti-pfmdr2 fusion protein. The slides containing fixed parasites were prepared from asynchronized W2 cultures as described in Materials and Methods. The cells were fixed with acetone and reacted with a rabbit anti-pfmdr2 fusion protein (dilution 1/50) followed by FITC-conjugated goat anti-rabbit IgG. (A) Light Microscopy. The arrow indicates a ring stage parasite. The trophozoite forms of infected erythrocytes can be identified by the hemozoin granules (dark crystals) within the digestive vacuole. (B) Fluorescent micrograph. The anti-pfmdr2 antibody only reacted with the trophozoite stage. the antibody staining appears to have a vacuolar localization.Zalis MG, Wilson CM, Zhang Y, Wirth DF. Characterization of the pfmdr2 gene for Plasmodium falciparum. Mol Biochem Parasitol. 1993 62:83-92.

See original on MMP

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