Immunoelectron microscopy showing the localization of PfRhop148 in the rhoptries within the developing merozoites in the schizont. Parasites were fixed with 1% paraformaldehyde and 0.1% glutaraldehyde. Staining was detected with rabbit anti-Rhop148 and anti-rabbit gold (10 nm). Bar represents 250 nm. IFA analysis showing stage-specific localization of PfRhop148. MAb 3C2 raised against the recombinant protein was used against staged synchronized parasites. Lower panels represent the merged image with DAPI dye.Lobo CA, Rodriguez M, Hou G, Perkins M, Oskov Y, Lustigman S. Characterization of PfRhop148, a novel rhoptry protein of Plasmodium falciparum. Mol Biochem Parasitol. 2003 128:59-65. Copyright Elsevier 2009.