Different PfRabs have different sub-cellular distributions in schizont-infected erythrocytes. Panel (A) shows control rabbit pre-immune serum. Panel (B) shows a multinucleated schizont decorated with anti-PfRab1A antibodies giving a perinuclear staining. Panels (C) and (D) (phase contrast) show the pattern obtained with the anti-PfRab11A antibodies. Clearly, PfRab1A and PfRab11A display different distributions. Panel (E) shows the pattern obtained with anti-Pf39 (ERC) antibodies specific for the endoplasmic reticulum. Panel (F) shows a double-labelling with anti-PfRab1A (green) and Pf39 (red) antibodies, where colocalization stains yellow. Panel (G) shows a double-labelling with anti-PfRab11A (green) and anti-Pf39 (red) and no co-localization is observed. Panel (H) shows anti-PfRab6 (green) and anti-Pf39 (red), where co-localization stains yellow.Quevillon E, Spielmann T, Brahimi K, Chattopadhyay D, Yeramian E, Langsley G. The Plasmodium falciparum family of Rab GTPases. Gene. 2003 306:13-25.

GFP-PfRab1A fluorescence is found as discrete loci in living cells. A. GFP fluorescence is observed as discrete loci termed PfRab1Abodies in trophozoite and schizont stage parasites expressing GFP-PfRab1A. B. GFP-PfRab18 fluorescence is diffuse and closely associated with the nuclei. C-terminal HA-tagged PfRab1A is distributed equally throughout the parasite cytoplasm.Morse D, Webster W, Kalanon M, Langsley G, McFadden GI. Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts. PLoS One. 2016 Jun 27;11(6):e0158174

GFP-PfRab1A fluorescence in late schizonts is associated with rhoptry markers. Rhoptry markers Rap1 and Ron4 are found in trophozoites and schizonts as discrete foci that colocalize with GFP-PfRab1A fluorescence in late schizonts, but not in earlier phases of parasite development.Morse D, Webster W, Kalanon M, Langsley G, McFadden GI. Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts. PLoS One. 2016 Jun 27;11(6):e0158174

GFP-PfRab1A fluorescence does not co-localize with ER/Golgi, dense granules or microneme markers. GFP-PfRab1A fluorescence is distinct from the localization of markers for the apicoplast (ACP), the ER (Bip), the Golgi (ERD2 and GRASP), as well as from staining of the ER/Golgi with Bodipy BFA. GFP-Rab1A fluorescence is also distinct from the localization of markers for dense granules (RESA) or micronemes (AMA1 and EBA175).Morse D, Webster W, Kalanon M, Langsley G, McFadden GI. Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts. PLoS One. 2016 Jun 27;11(6):e0158174