Localisation of LplA2-GFP was analysed by localising a C-terminally GFP-tagged protein in P. falciparum erythrocytic stages. Expression of the GFP-tagged protein in P. falciparum 3D7 was analysed by fluorescence light microscopy. The parasites were costained with MitotrackerTM Ros (Molecular Probes) to assess whether LplA2-GFP colocalises with the parasite’s mitochondrion. In panel 1 LplA2 is clearly colocalising with the mitochondrion. However, as is obvious from panel 2 LplA2-GFP localised also to an organelle close to the mitochondrion but clearly distinct from it. In panel 3 LplA2-GFP colocalises with the mitochondrion but also is present in a second organelle closely associated with the mitochondrion. Scale bars: 5 mm.Günther S, Wallace L, Patzewitz EM, McMillan PJ, Storm J, Wrenger C, Bissett R, Smith TK, Müller S. Apicoplast lipoic acid protein ligase B is not essential for Plasmodium falciparum. PLoS Pathog. 2007 Dec;3(12):e189

Immunofluorescent analyses were performed on P. falciparum 3D7 using anti-LplA2 and anti-aLipDH (aE3) antibodies (specifically staining the apicoplast of the parasites). Panel 1 shows staining of an organelle distinct from the apicoplast, probably being the mitochondrion. Panel 2 shows costaining of both antibodies, suggesting LplA2 is located in the apicoplast, and panel 3 shows staining of both the apicoplast and potentially the mitochondrion. Scale bars: 5 mm.Günther S, Wallace L, Patzewitz EM, McMillan PJ, Storm J, Wrenger C, Bissett R, Smith TK, Müller S. Apicoplast lipoic acid protein ligase B is not essential for Plasmodium falciparum. PLoS Pathog. 2007 Dec;3(12):e189.