Localization of PfBlm in different intra-erythrocytic stages of P. falciparum by immunofluorescence staining and confocal microscopy. The cells were fixed and immunostained with purified primary antibodies against PfBlm followed by fluoro-isothiocyanate-labelled secondary antibody and then counterstained with DAPI. In each panel, single confocal image of each stage is shown. (a) Ring stage, (b) Trophozoite stage, (c) Early schizont stage, (d) Schizont stage, (e) Double infection in schizont stage, (f) Bursting schizont stage. In each panel (i) phase contrast image; (ii) image of cell stained with DAPI (blue); (iii) immunofluorescently stained cell (green); (iv) super-imposed image; (v) merged.Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom omologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 Feb 23. [Epub ahead of print]

Co-localization of PfBlm and PfWrn by immunofluorescence dual colour staining and confocal microscopy. Panels a, b, c, d and e show the images of merozoite attached to RBC, trophozoite, early schizont, mature schizont and late schizont respectively. (i) Immunofluorescently stained cell (green, P. falciparum Wrn protein), (ii) immunofluorescently stained cell (red, PfBlm), (iii) super-imposed images of PfBlm and PfWrn, (iv) super-imposed images. The results reveal that PfWrn was found in nucleus as reported and PfBlm is expressed in nucleus as well as in cytoplasm. The co-localization of PfWrn and PfBlm was observed to some extent in invading merozoite (a) and trophozoite stages.Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 May;1864(5):594-608

Localization of PfBlmin different intra-erythrocytic stages of P. falciparum by immunofluorescence staining and confocalmicroscopy. The cells were fixed and immunostained with purified primary antibodies against PfBlm followed by fluoroisothiocyanate-labelled secondary antibody and then counterstained with DAPI. In each panel, single confocal image of each stage is shown. (a) Ring stage, (b) trophozoite stage, (c) early schizont stage, (d) schizont stage, (e) double infection in schizont stage, (f) bursting schizont stage. In each panel (i) phase contrast image; (ii) image of cell stained with DAPI (blue); (iii) immunofluorescently stained cell (green); (iv) super-imposed image; (v) merged. These data are representative of threeseparate experiments. The results showthat Blm protein expresses and localizesmainly in the nuclear region during the ring and trophozoite stages (a–b) but it is also detectable in cytoplasm during the schizont stages of intraerythrocytic development of the parasite.Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 1864(5):594-608.