"In D10- PfmtRPS18-3HA parasites, removal of aTc had negligible effect on both parasite growth and morphology (Figure 4C and Supplementary Small subunit proteins in the P. falciparum mitoribosome (Experimental Procedures). Although gold particles sometimes were present at other sites, the majority of them were found in the mitochondria at or very near the MIM (Figure 3A and 3B). In particular, quantification of gold particles from two biological experiments revealed the particles were localized to the mitochondria (at or near MIM) at 66.8%, the cytoplasm at 11.1%, the ER at 10%, the nucleus at 8.7% and others at 3.4%. Hence, our data indicate that 5 Downloaded from http://www.jbc.org/ by guest on April 11, 2020 Figure 4C), suggesting that PfmtRPS18 is not essential for asexual growth in vitro, consistent with the results from the recent large-scale piggyBac transposon mutagenesis survey, in which PfmtRPS18 was found to be mutable . "