Localization of PfFNT in infected erythrocytes. (a) Fluorescence pattern of live, PfFNT-GFP expressing early (top panel) and late trophozoites (bottom panel) within erythrocytes. DIC, differential interference contrast; DAPI (blue), nuclear stain; GFP (green). (b) A single confocal section through an infected erythrocyte. Bodipy-TX-ceramide (red), lipid membrane stain. (c,d) Co-localization in the plasma membrane of PfFNT-GFP with merozoite surface protein 1 (MSP1) by fluorescence microscopy (c) or a single confocal section (d) using anti-GFP and anti-MSP1 antibodies. Scale bars, 5 mm. Localization at the parasite’s periphery is consistent with a function of PfFNT in lactate and proton release.Wu B, Rambow J, Bock S, Holm-Bertelsen J, Wiechert M, Soares AB, Spielmann T, Beitz E. Identity of a Plasmodium lactate/H+ symporter structurally unrelated to human transporters. Nat Commun. 2015 6:6284