Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-5293 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-5294 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-5294 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-5309 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-5309 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-5310 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-5311 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Possible
RMgm-5318 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
37535401
Growth of the parasites was monitored over two continuous asexual replication cycles with Giemsa-stained thin smears prepared from in vitro cultures every 48 hours. Microscopic enumeration of parasitemias indicated that Pfeb1− parasites grow similarly to WT PfNF54 parasites : defect in nuclear segregation during microgametogenesis - Strikingly, however, examination of free microgametes for WT PfNF54 and Pfeb1− parasites for DNA staining revealed that the majority of Pfeb1− microgametes were DAPI negative
Theo Sanderson, Francis Crick Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7 Asexual
No difference
37535401
Growth of the parasites was monitored over two continuous asexual replication cycles with Giemsa-stained thin smears prepared from in vitro cultures every 48 hours. Microscopic enumeration of parasitemias indicated that Pfeb1− parasites grow similarly to WT PfNF54 parasites : defect in nuclear segregation during microgametogenesis - Strikingly, however, examination of free microgametes for WT PfNF54 and Pfeb1− parasites for DNA staining revealed that the majority of Pfeb1− microgametes were DAPI negative
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Gametocyte
Attenuated
37535401
Growth of the parasites was monitored over two continuous asexual replication cycles with Giemsa-stained thin smears prepared from in vitro cultures every 48 hours. Microscopic enumeration of parasitemias indicated that Pfeb1− parasites grow similarly to WT PfNF54 parasites : defect in nuclear segregation during microgametogenesis - Strikingly, however, examination of free microgametes for WT PfNF54 and Pfeb1− parasites for DNA staining revealed that the majority of Pfeb1− microgametes were DAPI negative
Theo Sanderson, Francis Crick Institute

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