BCDH and KDH localization (A) Epifluorescent images of live P. falciparum erythrocytic-stage parasites expressing GFP fused to either the BCDH leader peptide (E2-BCDH30) or the KDH leader peptide (E2-KDH30). The parasites were stained with MitoTracker to identify mitochondria and Hoescht to identify nuclei. Image z-stacks were deconvolved and then presented as a single combined image. In both cases, GFP fluorescence co-localizes with MitoTracker to the mitochondrion. Afanador GA, Matthews KA, Bartee D, Gisselberg JE, Walters MS, Freel Meyers CL, Prigge ST. Redox-dependent lipoylation of mitochondrial proteins in Plasmodium falciparum. Mol Microbiol. 2014 Aug 13. [Epub ahead of print]