Immunofluorescence analysis. Synchronized asexual parasites were stained with the immunopurified rabbit anti-Pfnek-1 antibody (top panels). The two bottom panels display negative controls using pre-immune serum. Scale bar: 5mm. Fluorescent dots are observed near the nuclei at the ring and schizont stages, whereas the pattern was more diffused and appeared as largely cytoplasmic in trophozoitesDorin-Semblat D, Schmitt S, Semblat JP, Sicard A, Reininger L, Goldring D, Patterson S, Quashie N, Chakrabarti D, Meijer L, Doerig C. Plasmodium falciparum NIMA-related kinase Pfnek-1: sex-specificity and assessment of essentiality for the erythrocytic asexual cycle. Microbiology. 2011 157(Pt 10):2785-94 Copyright Society for General Microbiology 2011.