Microscopy images of live parasites showing a ring stage (top), a trophozoite stage (middle) and a schizont stage parasite (bottom). Cellular location of PF3D7_1462300 (upper panel; PF14_0593 GTP-binding protein) and PF3D7_1310500 (lower panel conserved protein MAL13P1.50) endogenously tagged with GF. DIC, differential interference contrast; size bars: 5 μm. PF3D7_1462300 showed foci in schizont stage parasites and a weaker signal in ring stages that was not typical for PV. PF3D7_1310500 was located around the parasite’s nucleus, typical for an ER localization.Khosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print]