Mutants developed gametocytes and formed ookinetes in vitro in similar numbers to, and morphologically indistinguishable from, wild type parasites in Giemsa-stained preparations.Ookinetes do not develop into oocysts.After removal of the blood meal from dissected, gametocyte-infected Anopheles gambiae midguts, wild type ookinetes were found in the epithelium. In sharp contrast, no mutant ookinetes were observed. When dissected, infected A.stephensi midguts were examined for the presence of ookinetes or young oocysts on the basal surface, only wild type and never mutant parasites were observed. When ookinete motility was assessed by recording their positions on glass surfaces at 5 min time intervals, displacement of wildtype was observed but not mutant ookinetes, indicating that CTRP is involved in ookinete locomotion. The KO ookinetes were occasionally seen to straighten and bend.

Normal numbers of ookinetes are produced (in vitro and in vivo in A. stephensi). Ookinetes do not show any morphological differences from wild-type parasites with Giemsa staining under microscopic observation. Ookinetes do not develop into oocysts.

Ookinetes show reduced motility (complete defect in forward gliding motility)

Ookinetes are formed but no traversal through mosquito midgut cells. Motility of ookinetes is impaired.

Normal numbers of ookinetes are produced. Ookinetes do not develop into oocysts.

Normal numbers of ookinetes are produced. Ookinetes do not develop into oocysts.

Ookinetes show reduced motility (complete defect in forward gliding motility). No oocyst are formed.

Ookinetes are formed but no traversal through mosquito midgut cells. Motility of ookinetes is impaired.