Intracellular location of Pfd-COP and comparison with the locations of the ER-located protein, PfERC, the trans -Golgi marker, PfRab6, and the PV-located protein, Exp-1. (A) Erythrocytes infected with young trophozoite stage (top panels) or mature trophozoite stage (bottom panels) malaria parasites (FAC8 strain) were labeled with affinity purified rabbit anti-GST-Pfd-COP(90) antiserum followed by an FITC-conjugated anti-rabbit IgG. (B) Erythrocytes infected with trophozoite stage parasites (FAC8 strain) were labeled with affinity purified rabbit anti-GST-Pfd-COP(90) antiserum followed by an FITC-conjugated anti-rabbit IgG (green fluorescence) and a murine antiserum recognizing the ER-located protein, PfERC, followed by Alexa Fluor 568-conjugated anti-mouse IgG (red fluorescence). (C) Erythrocytes infected with trophozoite stage parasites (FAC8 strain) were labeled with affinity purified rabbit anti-GST-Pfd-COP(90) antiserum followed by an FITC-conjugated anti-rabbit IgG (green fluorescence) and a murine MAb recognizing the Golgi protein, PfRab6, followed by Alexa Fluor 568-conjugated anti-mouse IgG (red fluorescence). (D) Erythrocytes infected with trophozoite stage parasites (FAC8 strain) were labeled with affinity purified rabbit anti-GST-Pfd-COP(90) antiserum followed by an FITC-conjugated anti rabbit IgG (green fluorescence) and a murine MAb recognizing the PV located protein, Exp-1 PF11_0224, followed by Alexa Fluor 568-conjugated antimouse IgG (red fluorescence). Differential interference contrast (DIC) images are shown in the left hand panels. Overlays of the DIC and green fluorescence channels are shown in the right hand panels of (A). Overlays of the green and red fluorescence channels are shown in the right hand panels of (B-D). Optical slices were collected by confocal microscopy through the centre of the parasite. The bar in the top right hand panel represents 5 mm.Adisa A, Rug M, Foley M, Tilley L. Characterisation of a delta-COP homologue in the malaria parasite, Plasmodium falciparum. Mol Biochem Parasitol. 2002 123:11-21. Copyright Elsevier 2010.