Confocal microscopy of plasmodium stages immuno-stained with PfABCG antiserum. Immuno-histochemical staining of 3D7 cultured parasites with PfABCG antiserum. Columns 1-3 show PfABCG immuno-histochemical staining resolved with Alexa-fluor 488-labeled goat anti-rabbit secondary antibody (Green), counterstaining of nuclear DNA with DAPI (Blue), and differential interference contrast of infected erythrocyte, respectively. Rows A-D show early trophozoites, late trophozoites schizonts and female gametocyte stage V stained with PfABCG antiserum, respectively. Column 4 shows the merge of column 1 and 2, without Differential Interference Contrast (DIC). White arrowheads indicate the intensely stained structures beneath the parasite's plasma membrane.Edaye S, Georges E. Characterization of native PfABCG protein in Plasmodium falciparum. Biochem Pharmacol. 2015 Jul 31. [Epub ahead of print]

Co-localization of PfExp-1 and PfABCG in P. falciparum-infected erythrocytes. Double immuno-histochemical staining of infected erythrocytes at trophozoites stage with antibodies against PfABCG and PfExp-1 (Panel A) and PfCRT and PfExp-1 (Panel B). Column 1 shows the nuclear DNA staining with DAPI (blue). In columns 2 and 3, PfABCG signal (green) (A) and PfCRT signal (green) (B) are resolved with Alexa-fluor 488-labeled goat anti-rabbit secondary antibody, and PfExp-1 signal (red) (A and B) is resolved with Alexa-fluor 594-labeled goat anti-rabbit secondary antibody. Columns 4 and 5 show respectively the differential interference contrast (DIC) of infected erythrocyte and the merger of column 1, 2 and 3 without DIC. The digestive vacuole membrane staining of PfCRT does not overlap with PfExp-1 staining while the plasma membrane staining of PfABCG partially overlaps with the parasitophorous vacuole membrane signal of PfExp-1 as indicated by the arrow.Edaye S, Georges E. Characterization of native PfABCG protein in Plasmodium falciparum. Biochem Pharmacol. 2015 Jul 31. [Epub ahead of print] PMID: