P. falciparum encodes cytosolic and apicoplast tryptophanyl-tRNA synthetase isoforms. The first panel shows live cell fluorescence microscopy of TrpRSapi-GFP and live mitochondrial staining, while the second and third panels show IFAs of P. falciparum TrpRSapi-GFP and TrpRScyt-GFP in relation to the apicoplast marker ACP. Schematic representations of transfected plasmids are indicated above each panel. ACP, acyl-carrier protein; DIC, differential interference contrast.Pasaje CF, Cheung V, Kennedy K, Lim EE, Baell JB, Griffin MD, Ralph SA. Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum. Sci Rep. 2016 6:27531.

Loss of apicoplast in indolmycin-treated, IPP-rescued parasites. Live cell fluorescence comparing treated and untreated ACP-DsRed/CS-eYFP P. falciparum 3D7. ACP, apicoplast acyl-carrier protein. DIC, differential interference contrast. CS, mitochondrial citrate synthase. Pasaje CF, Cheung V, Kennedy K, Lim EE, Baell JB, Griffin MD, Ralph SA. Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum. Sci Rep. 2016 6:27531.