Two upper panels: Localisation of PfFtsH1 in a P. falciparum 3D7 transfectant line carrying C-terminal 3xHA-tagged PfFtsH1. (B) Immunofluorescence localization of PfFtsH1-HA using the anti-HA mAb and antibody against the apicoplast marker ACP. No overlap of PfFtsH1-HA signal was observed with the apicoplast marker. (C) PfFtsH1 co-localizes with the mitochondrial signal in trophozoites (upper panel) and appears as punctuate signals lining the organelle defined by the mitochondrial stain Mitotracker Red in schizonts (lower panel). (D) Confocal microscopy PfFtsH1-HA expressing parasites showing co-localisation of PfFtsH1 with the mitochondrion which is stained with Mitotracker Red.Lower panel: Localization of PfFtsH1 to the mitochondrion is confirmed by immunofluorescence with anti-FtsH1 Ab. Confocal immunofluorescence microscopy of P. falciparum 3D7 infected erythrocytes using Mitotracker Red and anti-FtsH1 Ab shows localization of PfFtsH1 in the parasite mitochondrion.Tanveer A, Allen SM, Jackson KE, Charan M, Ralph SA, Habib S. An FtsH protease is recruited to the mitochondrion of Plasmodium falciparum. PLoS One. 2013 8(9):e74408.