Normal production of male and female gametocytes. No motile gametes are formed after activation of male gametocytes.

Normal production of male and female gametocytes. No motile gametes are formed after activation of male gametocytes.

We first confirmed the importance of SAS6 in microgametogenesis as no exflagellation centres were observed 15 min post-activation in the SAS6-KO line, as previously described

Normal production of male and female gametocytes. No motile gametes are formed after activation of male gametocytes. Strongly reduced numbers of ookinetes (the few ookinetes formed have a normal morphology).

Normal production of male and female gametocytes. No motile gametes are formed after activation of male gametocytes. Strongly reduced numbers of ookinetes (the few ookinetes formed have a normal morphology).

Strongly reduced ookinete formation. Strongly reduced numbers of oocyst. Oocyst have a reduced size and aberrant DNA synthesis .

Strongly reduced ookinete formation. Strongly reduced numbers of oocyst. Oocyst have a reduced size and aberrant DNA synthesis .

Strongly reduced ookinete formation. Strongly reduced numbers of oocyst. Oocyst have a reduced size. No sporozoite formation

Strongly reduced ookinete formation. Strongly reduced numbers of oocyst. Oocyst have a reduced size. No sporozoite formation