PfROM4 is a merozoite plasma membrane protein. IFA of mature nonsegmented (top) and fully segmented (bottom) schizonts of 3D7HAROM4synth (parasites with episomal expression of N-terminal HA3-tagged PfROM4, under the control of the pfrom4 promoter), dual labeled with mAb 3F10 (anti-HA; green) and mAb 1E1 (anti-MSP1; red). The DAPI signal (blue) is not included in merged images for clarity. Bar, 5 μm.O'Donnell RA, Hackett F, Howell SA, Treeck M, Struck N, Krnajski Z, Withers-Martinez C, Gilberger TW, Blackman MJ. Intramembrane proteolysis mediates shedding of a key adhesin during erythrocyte invasion by the malaria parasite. J Cell Biol. 2006 174:1023-33.