(F) Localization of PfSSB by immunofluorosecnce analysis using glass slide containing fixed parasites obtained from trophozoite and schizont stages in the presence of anti-PfSSB antibodies. DAPI indicates nuclei whereas merged panel shows the presence of PfSSB with respect to the nuclei. The last panel shows the phase picture of parasite infected RBC. G) Immuno-colocalization of PfSSB and apicoplast marker acyl carrier protein (ACP). Merged panel shows the co-localization of both the proteins. Distinct subnuclear spot corresponding to apicoplast was observed in late ring/trophozoite stage parasites with undivided nucleus (F, first row, top panel,) whereas multiple subnuclear spots were observed in multinucleated schizont stage parasites (F, bottom two rows, top panel). These results are consistent with the multiplication of apicoplast organelle with parasite growth.Prusty D, Dar A, Priya R, Sharma A, Dana S, Choudhury NR, Rao NS, Dhar SK. Single-stranded DNA binding protein from human malarial parasite Plasmodium falciparum is encoded in the nucleus and targeted to the apicoplast. Nucleic Acids Res. 2010 38(20):7037-53 Copyright Elsevier 2011