Localization of aminopeptidases in P. falciparum blood stages.PfAPP Aminopeptidase P; PfA-M1 Aminopeptidase N; PfLAP Leucyl aminopeptidase; PfDAP Aspartyl aminopeptidase B, Epifluorescence microscopy of live parasites expressing fusions of YFP with the indicated aminopeptidases. YFP fluorescence is pseudocolored green and Hoechst 33342 (DNA) fluorescence is pseudocolored red. The arrowhead indicates the food vacuole. Bar, 2 mm. C, indirect immunofluorescence detection of PfA-M1-HA. Secondary antibody fluorescence is pseudocolored green and DAPI (DNA) fluorescence is pseudocolored red. Bar, 2 mm.Dalal S, Klemba M. Roles for two aminopeptidases in vacuolar hemoglobin catabolism in Plasmodium falciparum. J Biol Chem. 2007 282:35978-87.

Cytochemical localization of the P. falciparum M18AAP. A, indirect immunofluorescence of D10 wild-type parasites probed with polyclonal antisera to native PfM18AAP. B, indirect immunofluorescence of PFI1570c-GFP transgenic parasites probed with specific antisera to GFP. C, direct fluorescence on live PFI1570c-GFP transgenic parasites. Parasite nuclei were visualized with Hoechst dye (blue). Specific staining was observed in the cytosol of the ring and schizont stage parasites (green, fluorescein isothiocyanate).Teuscher F, Lowther J, Skinner-Adams TS, Spielmann T, Dixon MW, Stack CM, Donnelly S, Mucha A, Kafarski P, Vassiliou S, Gardiner DL, Dalton JP, Trenholme KR. The M18 aspartyl aminopeptidase of the human malaria parasite Plasmodium falciparum. J Biol Chem. 2007 282:30817-30826.