Immunofluorescence assays for localization of PfRRF2.A. Live cell imaging of the PfRRF2leader–GFP transfectant with MitoTracker indicates predominantly mitochondrial targeting of PfRRF2. C. Immunofluorescence localization using anti-GFP Ab and MitoTracker shows colocalization of the PfRRF2leader–GFP signal with mitochondrion.D. Absence of overlap between the apicoplast marker ACP and PfRRF2leader–GFP indicating that the PfRRF2 leader does not target the protein to the apicoplast. E. Immunofluorescence localization of PfRRF2 in P. falciparum 3D7-infected erythrocytes using rabbit anti-RRF2 antibody as probe. Cellswere stained with MitoTracker prior to fixation. F. P. falciparum D10 ACPleader–GFP infected erythrocytes were probed with anti-GFP and anti-RRF2 Abs. PfRRF2 signal did not overlap with the apicoplast.Gupta A, Mir SS, Jackson KE, Lim EE, Shah P, Sinha A, Siddiqi MI, Ralph SA,Habib S. Recycling factors for ribosome disassembly in the apicoplast andmitochondrion of Plasmodium falciparum. Mol Microbiol. 2013 88(5):891-905 .