Localisation of the PfRON6 protein in parasitised red blood cells. (B) Double labelling using anti-rPfRON6-B and anti- haemagglutinin (HA) in HA-tagged transgenic parasites. Confocal microscopy using anti-rPfRON6-B with anti-HA. Corresponding overlay images are shown. (C) Double labelling of PfRON6 with Pf34, PfRON4, RAMA or AMA1 in segmented schizonts. Confocal microscopy using anti-rPfRON6-B with anti-Pf34 (rhoptry neck marker), anti-PfRON4 (rhoptry neck marker), anti-RAMA (rhoptry bulb marker), or anti-AMA1 (microneme marker). Corresponding overlay images are shown. a punctate pattern of fluorescence in segmented schizonts characteristic of localisation with the apical secretory organelles.Proellocks NI, Kats LM, Sheffield DA, Hanssen E, Black CG, Waller KL, Coppel RL. Characterisation of PfRON6, a Plasmodium falciparum rhoptry neck protein with a novel cysteine-rich domain. Int J Parasitol. 2008 39(6):683-92. Copyright Elsevier 2009.

Upper 2 rows: Localization of Pf34 to the rhoptries was demonstrated in late stage parasites by immunostaining infected blood smears with anti-Pf34 and co-localising with either anti-RAMA or anti-RhopH3 (both rhoptry markers) or anti-AMA-1 (microneme marker) antibodies.Lower row: A mature schizont (i) and two extracellular merozoites (ii) showing the close apposition of Pf34-positive (green) and RAMA-positive (red) structures in the apex of the merozoite. Panels (iii) and (iv) show the apex of merozoites and the two rhoptries connected to the Pf34-positive structure at the anterior when visualised by confocal microscopy. N, nucleus; R, rhoptry. Bars represent 1 mm in (i) and (ii) and 500 nm in (iii) and (iv).Proellocks NI, Kovacevic S, Ferguson DJ, Kats LM, Morahan BJ, Black CG, Waller KL, Coppel RL. Plasmodium falciparum Pf34, a novel GPI-anchored rhoptry protein found in detergent-resistant microdomains. Int J Parasitol. 2007 37:1233-41. Copyright Elsevier