PfRON5 protein localization. PfRON5 is expressed only in schizonts and free merozoites PfRON5 displays a rhoptry localization pattern on the IFA (arrows show the punctate pattern in the hyper-immune serum sample). Hyper-immune serum (HI). Pre-immune serum (PI).Curtidor H, Patiño LC, Arévalo-Pinzón G, Patarroyo ME, Patarroyo MA. Identification of the Plasmodium falciparum rhoptry neck protein 5 (PfRON5). Gene. 2010 474:22-8. Copyright Elsevier 2011

The PfRON4 and PfRON5 proteins localize to the rhoptry neck. A, Antibodies raised against PfRON4 and PfRON5 recognize specific protein products in schizont parasite extracts. B, IFAs using the anti-PfRON antibodies reveal staining of the apical tip of free merozoite, in close apposition with the rhoptry bulb markers PfRAP1 and PfRAMA, suggesting a rhoptry neck localization. Scale bar: 0.2μm C, Immunoelectron microscopy confirms that PfRON 4 localizes to the rhoptry neck. Scale bar: 0.1μmRichard D, Macraild CA, Riglar DT, Chan JA, Foley M, Baum J, Ralph SA, Norton RS, Cowman AF. Interaction between Plasmodium falciparum apical membrane antigen 1 and the Rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites. J Biol Chem. 2010 285:14815-22

The PfRON4 and PfRON5 proteins localize to the rhoptry neck. A, Antibodies raised against PfRON4 and PfRON5 recognize specific protein products in schizont parasite extracts. B, IFAs using the anti-PfRON antibodies reveal staining of the apical tip of free merozoite, in close apposition with the rhoptry bulb markers PfRAP1 and PfRAMA, suggesting a rhoptry neck localization. Scale bar: 0.2μm C, Immunoelectron microscopy confirms that PfRON 4 localizes to the rhoptry neck. Scale bar: 0.1μmRichard D, Macraild CA, Riglar DT, Chan JA, Foley M, Baum J, Ralph SA, Norton RS, Cowman AF. Interaction between Plasmodium falciparum apical membrane antigen 1 and the Rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites. J Biol Chem. 2010 285:14815-22

PfRON5 localizes to the rhoptry neck. (A) Aotus antibodies against MSP-1 peptide 24148 (5501) displaying the membrane, and against RAMA peptide 36340 (33460),showing the rhoptries (red fluorescence), together with RON5 peptide 38556 (37742) (green fluorescence) and DAPI (blue fluorescence, merged at the bottom), confirming RON5 localization in the rhoptries but differently to that of RAMA. (B) Immuno-electron microscopy with rabbit antibodies against RON5 peptides and protein A-gold showed specific labeling in the P. falciparum merozoite rhoptry neck. PfRON5 was detected in pear-shaped rhoptry neck portion in segmented schizonts when using rabbit polyclonal antibodies for IEM assay.Curtidor H, Patiño LC, Arévalo-Pinzón G, Vanegas M, Patarroyo ME, Patarroyo MA. Plasmodium falciparum rhoptry neck protein 5 peptides bind to human red blood cells and inhibit parasite invasion. Peptides. 2013 Aug 8. 9781(13)00268-4.