Immunofluorescence localization of PfIF3a and PfIF3b. (B) Panel i, P. falciparum-infected erythrocytes stained with Mitotracker Red and probed with PfIF3a antiserum; panel ii, 3-dimensional reconstruction after z-sectioning shows clear overlay of the PfIF3a signal with the mitochondrion; panel iii, Absence of overlap of the PfIF3a signal with apicoplast PfHU. (C) Panel i, parasitized erythrocytes probed with anti-IF3b and anti-HUp anti-sera; panel ii, 3-dimensional reconstruction shows overlap of IF3b with apicoplast HUp; panel iii, PfIF3b signal does not overlap with Mitotracker Red. Nuclear DNA is stained with DAPI.Haider A, Allen SM, Jackson KE, Ralph SA, Habib S. Targeting and function of proteins mediating translation initiation in organelles of Plasmodium falciparum. Mol Microbiol. 2015 Feb 17. [Epub ahead of print]