Immunofluorescence studies performed using anti-PfPKB antisera on thin blood smears of P. falciparum cultures revealed that only late schizonts but no other asexual stages (Hoechst-stained) were recognized by this anti-sera. Upper row: Rabbit antisera against PfPKB (red) and mouse antisera against MSP1 (green) was used to localize these proteins in late/segmented schizonts, or in free merozoites (lower row). The right panel in the lower row shows a zoomed image of merozoites stained with MSP1 and PfPKB antisera. PfPKB exhibited a diffused staining pattern in segmented schizonts, indicating its presence in the cytoplasm, and also exhibited some co-localization with MSP1. In free merozoites PfPKB seems to be localized at the apical end.Kumar A, Vaid A, Syin C, Sharma P. PfPKB, a novel protein kinase B-like enzyme from Plasmodium falciparum: I. Identification, characterization, and possible role in parasite development. J Biol Chem. 2004 279:24255-64.