Upper panels: The intense fluorescent focus of FLN-GFP appears to partially overlap with that of the apicoplast. Clone MP3 parasites were fixed with 4% paraformaldehyde/0.0075% glutaraldehyde and stained with rabbit anti-ACP antibody, a marker for the apicoplast lumen (red) and goat anti-GFP antibody (green). Cells were observed by epifluorescence microscopy. (i–iv) Representative images of cells at ring, trophozoite, schizont and segmenter stages respectively. LOwer panel: FLN–mitochondrion association. Clone MP3 was examined by IFA and epifluorescence microscopy using rabbit anti-Hsp60 (red) and goat anti-GFP (green) antibodies. (i–iv) Representative images of cells at ring, trophozoite, schizont and segmenter stages respectively.Ponpuak M, Klemba M, Park M, Gluzman IY, Lamppa GK, Goldberg DE. A role for falcilysin in transit peptide degradation in the Plasmodium falciparum apicoplast. Mol Microbiol. 2007 63:314-34

Expression of DsRED and GFP in Plasmodium falciparum double transfectants. Localization of fluorescent proteins DsRED (red) and GFP (green) in pSSPF2/PfHsp60-DsRED–pSSPF2/PfACP-GFP double transfectant (A), pSSPF2/PfACP-DsRED–pSSPF2/PfHsp60-GFP double transfectant (B) and pSSPF2/PfCpn60-DsRED–pSSPF2/PfACP-GFP double transfectants (C–E) was monitored by fluorescence microscopy. +DiC, overlay on a phase contrast image with the blue signal of Hoechst 33342 indicating the position of the nucleus of the parasites. (A, B) Schizont; (C) two co-infecting ring stage parasites; (D) trophozoite; (E) schizont. Scale bar: 5 mm.Sato S, Wilson RJ. The use of DsRED in single- and dual-color fluorescence labeling of mitochondrial and plastid organelles in Plasmodium falciparum. Mol Biochem Parasitol. 2004 134:175-9. Copyright Elsevier