Indirect immunofluorescence images of P. falciparum schizonts stained with rabbit IgG (green) induced by 10 viral-vectored vaccines expressing malaria antigens, and negative control vectors lacking a malaria antigen. Nuclei are counterstained with 4,6-diamidino-2-phenylindole (blue). All sera were tested against 3D7 strain parasites, with the exception of anti-PfRH1 for which FVO strain parasites were used. All images to same scale as Giemsa stained image (top left, on which scale bar indicates 5 μm).Douglas AD, Williams AR, Illingworth JJ, Kamuyu G, Biswas S, Goodman AL, Wyllie DH, Crosnier C, Miura K, Wright GJ, Long CA, Osier FH, Marsh K, Turner AV, Hill AV, Draper SJ. The blood-stage malaria antigen PfRH5 is susceptible to vaccine-inducible cross-strain neutralizing antibody. Nat Commun. 2011 2:601.

Subcellular localization of MSP3 and ABRA Smears of late schizonts (s) of anti-MSP1 (located on the surface of the merozoite (m)) and anti-MSP3 or anti-ABRA antibodies. Anti-MSP1 mouse antibodies were labeled with rhodamine-conjugated anti-mouse antibodies (red). Anti-MSP3 and anti-ABRA rabbit antibodies were labeled with anti-rabbit antibodies conjugated with FITC (green). Co-localization is shown in the row and indicated by yellow. D10 smears labeled with anti-MSP1 (first column) and anti-MSP-3 or anti-ABRA (second column). In late D10 schizonts, MSP-3, MSP-1 and ABRA had a localization consistent with the parasitophorous vacuole and the merozoite surface. Mills KE, Pearce JA, Crabb BS, Cowman AF. Truncation of merozoite surface protein 3 disrupts its trafficking and that of acidic-basic repeat protein to the surface of Plasmodium falciparum merozoites. Mol Microbiol. 2002 43:1401-1411. Copyright John Wiley & Sons Ltd. 2010.

P113 is expressed in schizonts and on the surface of free merozoites. Fixed blood-stage P. falciparum schizonts and free merozoites were probed with anti-P113 antibodies (green), the merozoite surface marker anti-MSP9 (red) and nucleic acid stained with DAPI (blue). Scale bars, 3 mm. P113, is expressed in early and late-stage schizonts and on the surface of free merozoites by co-staining with the established merozoite surface markers MSP9.Galaway F, Drought LG, Fala M, Cross N, Kemp AC, Rayner JC, Wright GJ. P113 is a merozoite surface protein that binds the N terminus of Plasmodium falciparum RH5. Nat Commun. 2017 8:14333. PMID: 28186186